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KMID : 0900919930170040375
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Korean journal of Animal Reproduction
1993 Volume.17 No. 4 p.375 ~ p.383
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Secretion of Human Growth Hormone from Mammary Gland of Transgenic Mice
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Lee Hoon-Taek
Lee Kyung-Kwang
Lee Sang-Min
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Abstract
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The human growth hormone (hGH) gene uder the control of the rat beta-casein promoter gene was designed to produce transgenic mouse expressed hGH gene in only mammary gland. One hundred seventy two eggs microinjected were transferred to the oviducts of pseudopregnants and 43 offspring were delivered. By Southern blotting hybridization, 3 were transgenic with rat beta-casein/hGH gene. The copy numbers of three transgenic founder were 1, 5, and 15, respectively. A radioimmunoassay was developed to quantitate the amount of expression of the hGH gene in mammary gland of transgenic mice. The amount of hGH was 13.3ng/ml in the lactating milk of one transgenic line, showing predominantly higher than 3.0ng/ml in milk of control mice. Therefore, our findings suggested that beta-casein promoter may induce the tissue specific expression of structural gene.
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